A strategy for seamless cloning of large DNA fragments fromStreptomyces
نویسندگان
چکیده
منابع مشابه
A strategy for seamless cloning of large DNA fragments from Streptomyces.
We report a novel method for the seamless cloning of large DNA fragments (SCLF) of up to 44 kb or larger from Streptomyces chromosomal DNA. SCLF is based on homologous recombination in Streptomyces and is easy to perform. The strategy of SCLF is to flank the target sequence in the chromosomal DNA with two identical restriction sites by the insertion of plasmids containing that site at either en...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2015
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114338